An investigation into how concentration of enzyme affects rate of reaction
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Sc1 - An investigation into how concentration of enzyme affects rate of reaction AIM The aim of this investigation is to see how different levels of enzyme concentration affect the rate of an enzyme controlled reaction. In this case, the substrate is pectin, found in the cell walls of apples, and the enzyme is pectinase. VARIABLES The variables in this experiment are: - temperature - pH - concentration of enzyme - concentration of substrate - time - inhibitors The chosen variable is concentration of enzyme. HYPOTHESIS I predict that as the concentration of enzyme increases, the rate of the reaction will also increase. This will be in a directly proportional ratio, until a peak rate of reaction occurs where the rate cannot increase any further. This will produce a straight-line graph through the origin, which will eventually flatten off at peak rate. This is what I estimate the graph to look like: Transmission of light (%) Enzyme conc. This hypothesis can be...


