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Matthew Scott 04/11/01 An Investigation of the Effect of Copper Sulphate on Catalase Activity Introduction Catalase is an enzyme which catalyses the reaction between hydrogen peroxide (H2O2) is broken down into oxygen (O2) and water (H2O). catalase 2H2O2 2H2O + O2 The heavy metal ions Cu+2 in copper sulphate inhibit this reaction. Copper sulphate is an irreversible non-competitive inhibitor. Scientific Knowledge Five main factors effect enzyme controlled reactions. They are: temperature; pH; substrate concentration; enzyme concentration; and, whether or not inhibitors are present. Therefore to investigate the effect of copper sulphate on catalase activity the temperature, pH, substrate concentration and enzyme concentration must be kept constant throughout the experiment. The factor to be investigated is the addition of inhibitors as it is the only factor to be varied. Enzymes are protein molecules, which act as biological catalysts. They contain an active site to which a molecule or molecules can bind. This molecule is known as the substrate of...

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