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Investigation to compare quantitatively the concentration of reducing sugars in samples of fresh orange, lemon and grapefruit juice Introduction - Background Knowledge This experiment involves the test for reducing and non-reducing sugars, using the Benedict's test. When a reducing sugar is heated with an alkaline solution of copper II sulphate (Benedict's solution), it forms an insoluble precipitate of copper I oxide. The colour of the precipitate then changes from green through yellow, orange and brown to deep red, depending on the quantity of reducing sugar present. Any substance providing electrons to carry out reduction is called a reducing agent, which reduces other substances. Benedict's reagent contains copper (II) sulphate. The copper two plus ions from the copper sulphate are reduced by electrons from the (C double bond O or a carbonyl group) found in certain sugars to form copper one plus ions. A resulting changes to copper (I) oxide to form a...

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