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What effect do different concentrations of bile have on the lipase activity?
Member rating: No Rating | Words: | Submitted: Fri Mar 31 2006
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Problem: What effect do different concentrations of bile have on the lipase activity? Experimental Design: Lipase is a digestive enzyme commonly used in our digestive system. There are several types of lipase including pancreatic lipase (mostly) and salivary lipase. Bile is a solution containing negatively charged bile salt, which separates the insoluble lipid into smaller pieces and kept them small (emulsification). To test different concentrations on the lipase activity, obviously more than one different concentration of bile solution is used and lipase concentration and volume are controlled. To isolate the effects of different [bile] on lipase activity as a single variable, any other variables effecting lipase activity would be controlled. The source of fat is cream and volume/concentration of sodium bicarbonate must be the same in each individual trial. It is entirely possible that different volumes of phenolphthalein would effect the pink intensity of individual test tubes, thus in caution,...
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