Logged out
Access All our Essays in an Instant
Swap your work for FREE access, or pay £4.99 for instant access
Find out how temperature affects the enzymic action of urease on urea to produce Carbon dioxide and ammonia.
Member rating: No Rating | Words: | Submitted: Tue May 25 2004
Page Preview
On the left is an image preview of every page of this document, and below are the first 150 words with formatting removed:
My investigation I have carried out a research on the type of enzyme that I have chosen to use, urease. Urease is an enzyme that catalyses the hydrolysis of urea into ammonia and carbon dioxide. The reaction occurs as follows: It is a globular protein found in bacteria and several higher plants as found by James Sumner in 1926. Its molecular weight is 480kDa with optimum pH of 7.4 and its enzymatic specificity is urea and hydroxyurea. Urea is the chief solid component of mammalian urine; synthesized from ammonia and carbon dioxide and used as fertilizer, in animal feed and in plastics. Preliminary Work I have carried some preliminary experiment to help me decide on how to measure measure the effect of temperature on the activity of urease. In the first experiment, I used the universal indicator to find out if temperature has on effect on the pH of the ammonium produced by...
Get instant access
- Instant, unlimited access to our documents in full
- Swap your work for free access, or pay £4.99
- To see the full version of this document and 147,383 others
Receive email updates for this category
- Simply tell us your email address and receive a weekly Study Help Email for FREE
- Receive 3 FREE essay views with each email
- Get all the latest essays from Coursework.Info & discussion from TheStudentRoom.co.uk
