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Egg albumen experiment.

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1564
Submitted:
Mon Jun 28 2004
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Have a little read: ... contents page INTRODUCTION 3 APPARATUS LIST 3 METHOD 4 fig 1: serial dilution table 4 RESULTS 5 Fig 2: results table 5 Fig 3: table of averages 5 Fig 4: graphical representation of the data. 6 DISCUSSION 7 EVALUATION 7 APPENDIX 8 Albumen information 8 The peptide bond 8 Introduction The purpose of this investigation is to establish which is the lowest concentration of Copper (II) Sulphate solution that will denature a sample of egg albumen (egg white) at room temperature. The base of the reaction is the globular protein (albumen) being denatured by a heavy metal (Copper (II)), the copper (II) reacts with the NH3 group causing it to denature, this means the proteins' secondary and tertiary structures are being altered and refolding into different shapes, this resulting in a change from the substance being clear to turning opaque.1 As the concentration of the denaturants increases more folding and changing of shape will occur and therefore more denaturing will occur and at a faster rate. From this I can predict that that lowest concentration of the

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