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The aim of this investigation is to investigate how changing the temperature affects growth and survival of bacteria. The bacterium’s Escherichia coli, Bacillus stearothermophilus, Bacillus subtilis, Pseudomonas fluorescens and Enterococcus
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Student: N009834 The Effect of Heat on Growth and Survival of Bacteria Abstract The aim of this investigation is to investigate how changing the temperature affects growth and survival of bacteria. The bacterium's Escherichia coli, Bacillus stearothermophilus, Bacillus subtilis, Pseudomonas fluorescens and Enterococcus faecalis will be investigated. Each of the bacteria were loop inoculated into glucose nutrient broths, then incubated at five different temperatures, 4OC, 25OC, 37OC, 45OC and 55OC for seven days to see where growth, if any has occurred at which particular temperature. Results showed that bacterium Pseudomonas fluorescens grew best at the lower temperatures and Bacillus subtilis grew paramount at the higher temperatures. Another experiment was carried out where the aim of the investigation was to show the relationship between the temperature and the time to kill for a number of species of bacteria. Each of the bacterium's nutrient broth bottles were placed in four waterbaths at...
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