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Liquid culture (E.coli in this case) is diluted to reduce the number of organisms per unit volume. This is necessary when numbers are being counted, and counting too may organisms may be inaccurate. Serial dilutions are made
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Serial dilution factor Introduction Liquid culture (E.coli in this case) is diluted to reduce the number of organisms per unit volume. This is necessary when numbers are being counted, and counting too may organisms may be inaccurate. Serial dilutions are made by transferring known standard volume of distilled water. This is used for several times to give a range of dilutions. The most usual factor is 10, giving a set of dilutions 10 , 10 , and so on, depending on initial culture concentration. Dilutions greater than 10-6 are rarely necessary. * Serial dilutions are used by environment offices, research and forensic science. Aim:- The aim of serial dilutions is used for investigating the identity of the original population of the sample. Apparatus 10 test-tubes, pipette, labelling tape, nutrient/macconkeys agar, test-tube holders, spreader, Bunsen burner, alcohol Method * Prepare a serial dilutions * Transfer 9cm of distilled water into 2 cm each of the series...
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