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Investigating the effect of four antibiotic agents on gram positive and gram negative bacteria Aim To find out how four antibiotic agents- Penicillin G, Streptomycin, fresh garlic and odourless garlic- effect gram-positive and gram-negative bacteria. The two different bacteria used will be Bacillus subtilis and Escherichia coli. After transferring the antibiotics to the bacteria I will be able to look for zones of inhibition in the bacteria where the antibiotic agents were placed. Scientific knowledge & understanding Bacteria are prokaryotes, which means they are organisms that lack a nuclei and are a lot smaller in volume than Eukaryotes (animals, plants, fungi & protoctists), on average only 0.5-5 m in diameter. Bacteria are a lot simpler in their structure. Structures always found in bacteria include a cell wall, plasma membrane, cytoplasm, ribosomes and circular DNA. Other structures that are present in some bacteria can be shown in the diagram below. Bacteria can be found almost anywhere...

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