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Investigating the enzyme ‘Catalase’  

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Year 11 Coursework: Investigating the enzyme 'Catalase' AIM To look at Hydrogen Peroxide (H2O2) being broken down into H2O and Oxygen (O2). We will also investigate the factors that change the rate of reaction. MATERIALS Scalpel White tile 30cm clear plastic ruler Test tubes & rack Stopclock 10ml measuring cylinder 2ml pipette H2O2 Cylinder of potato Goggles Cloth SAFETY The hydrogen peroxide is toxic, and is a bleaching agent therefore if it is spilled it must be cleaned up straight away. You must wear safety goggles to protect your eyes. The scalpel is dangerous, it is very sharp so you must not run and also be very careful while handling a scalpel. METHOD (pilot experiment) 1) We will measure out the desired amount of H2O2 and pour it into a test tube 2) We mark and cut out the desired amount of potato with a scalpel. 3) Every 4 minutes we will measure the height of froth produced, to 1 decimal point, from the meniscus. 4) Record results 5) Plot a graph...

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