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To investigate the effect of enzyme concentration on the activity of catalase in potato tuber cells on hydrogen peroxide measuring the time taken to produce 5 ml of oxygen produced to determine the rate of reaction.
Member rating: No Rating | Words: | Submitted: Wed Aug 27 2003
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Aim: - To investigate the effect of enzyme concentration on the activity of catalase in potato tuber cells on hydrogen peroxide measuring the time taken to produce 5 ml of oxygen produced to determine the rate of reaction. Method: - 1. Set up apparatus as shown in the diagram below. 2. Using the measuring cylinder add 50 cm3 20vol. hydrogen peroxide solution into the conical flask. 3. Use the cork borer to bore several cylinders from the potato all of equal thickness. Cut the potato cylinders into a length of 4 cm (ensure there is no potato skin present). You will need to cut 45 potato cylinders in total. Make sure you cut all the potato cylinders with the same cork borer and measure the diameter of the potato cylinder to work out the surface area. 4. Ensure your apparatus is set up correctly, that the graduated cylinder is inverted and full of water and placed...
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