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The aim of this experiment is to demonstrate the effects of pH on potato catalase. My substrate is hydrogen peroxide and the enzyme catalase will be from three types of potatoes.
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Aim The aim of this experiment is to demonstrate the effects of pH on potato catalase. My substrate is hydrogen peroxide and the enzyme catalase will be from three types of potatoes. INTRODUCTION Enzymes are an important part of all living organisms in that they regulate chemical reactions within the organism. Enzymes are biological catalyst made of globular protein that speed up a reaction by lowering the activation energy while at the same time they do not take part of it. Because of enzyme's tertiary arrangement they are specific in the job they do and they can be altered by high temperatures, high pH and also by irreversible inhibitors. Enzyme denaturation is a result of the breaking of the hydrogen bonding, ionic bonding and/or the disulphide bonding which hold the enzyme molecule in its three dimensional shape. The shape that is altered is the active site which fits into a specific substrate....
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