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The Effect of pH on Catalase Hypothesis: The rate at which catalase catalyses the decomposition of hydrogen peroxide increases to an optimum pH and then decreases as the optimum pH is exceeded. Biological knowledge: Hydrogen peroxide is produced in plant and animal cells as a by-product, which is toxic, resulting in the need of catalase to speed up the decomposition of hydrogen peroxide to water and oxygen. All microbodies contain catalase and it is the fastest known enzyme with a turnover number of 6 million. It is especially abundant in plant storage organs and in liver. It is effective over the pH range of 4.0 to 9.0 with the optimum pH of 7.6. 2H2O2(aq) catalase 2H2O(l) + O2(g) As an enzyme, its rate is affected by various factors, including pH. Changes in pH alter the ionic charge of acidic and basic groups of the enzyme. This therefore distorts the shape of the...

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