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Observe how much oxygen is given off, when we change the concentration of catalase in potato discs, during the reaction with hydrogen peroxide.
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| Submitted: Mon Jan 12 2004
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Catalase Investigation In this investigation we wanted to observe how much oxygen is given off, when we change the concentration of catalase in potato discs, during the reaction with hydrogen peroxide. Related knowledge about catalase * The temperature and pH affect catalase activity * It works best at the pH 7 - and room temperature * Catalase becomes denatured if subjected to high temperature or pH other than 7 * Affects active site shape activity Apparatus * Scalpel * Ruler * White tile * Tweezers * Test tube with side arm and tubing * Delivery tube * Retort stand * Bung * Water bath * Potato Cylinders * 20% Hydrogen peroxide * 2 measuring cylinders * Stop clock * Bucket for waste Prediction My prediction is that the greater the catalase concentration, the more oxygen will be collected over 5 minutes. The more pieces of potato you put in (or the higher the surface area) the bigger the result will be. Link to theory - More Catalase means more active site, this means more...
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