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Investigation on how pH affects free and immobilised catalase enzymes Aim My aim in this experiment is to find out whether the reaction rate of particular enzymes more affected by pH 4.4, 5.2, 6.5, 7.0, or 7.5 more when it is immobilised by using sodium alginate to trap it in beads or just used as free enzymes. And from this I can compare whether the reaction rate differs most in free or immobilised. Research Catalase occurs in many plant and animal tissue. It breaks down toxic Hydrogen peroxide, formed as a by-product of various biochemical reactions, into water and oxygen. The activity of most enzymes is influenced by changes in pH. In this experiment, potato discs in solutions of known pH act on hydrogen peroxide, and the rate at which oxygen is evolved is measured. This reflects the activity of the Catalase in the potato. Each enzyme has an optimal Ph, which...

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