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Biology Coursework. Investigating the influence of pH on the activity of POTATO CATALASE Conclusion. Enzymes are globular proteins which can be denatured at extreme pH levels The active site is a region of the protein/enzyme such as a cleft or crevice in the tertiary structure of the enzyme, often constituting less than 5% of the surface area. The active site can be negatively charged to attract for example a positively charged amino group (basic side chains) and vice versa, namely positively charged to attract for example a carboxylic group. As the hydrogen ion concentration decreases the pH number increases and a solution becomes more alkaline / basic. Amino acids are amphoteric having both a basic (amine) group and acidic (carboxylic acid) group). In water, the carboxylic acid partially dissociates becoming negatively charged and similarly the amine group accepts a hydrogen ion becoming positively charged. In this state, an amino acid becomes a...

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