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Investigating the influence of pH on catalase
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| Submitted: Mon Aug 18 2003
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James Fung 3rdDecember, 2001 Biology investigation - Investigating the influence of pH on catalase Introduction: I am going to investigate the effect of reaction rate and the influence of pH on catalast found in potatoes. Background information: Hydrocarbon peroxide is a toxic substance formed during aerobic respiration. It is removed by the enzyme, catalase which is found in many plants and animal tissue. Catalase catalyses the breakdown of hydrogen peroxide into water and the gas oxygen 2H2O2 (l) 2H2O (l) + O2 (g) Equipment and substances needed: 1) Potato 2) Cork borer 3) Scalpel 4) Ruler 5) Ceramic tile 6) Measuring cylinders 7) Stopwatch 8) Gas syringe 9) Conical flask with a lid 10) Citric-acid phosphate buffers (pH3, pH4, pH5, pH6, pH7, pH8) 11) 20 volume of hydrogen peroxide 12) Clamp stands Method and steps: 1) Link a conical flask with a gas syringe and use the clamp stand to hold the gas syringe 2) Cut 20 pieces of potato of 5 mm to 5 mm in size by using the...
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