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Investigating the Effect of Substrate Concentration on Catalase Aim To investigate the effect of substrate concentration the enzyme catalase using the decomposition reaction of hydrogen peroxide and measuring the volume of oxygen produced. Hypothesis Enzymes are globular protein molecules, which catalyse chemical reactions and therefore speed up the rate of the reaction and yet remain unchanged (Jones, Fosbery and Taylor, 2000). The enzyme's structure, as it is three-dimensional, is determined by the sequence of amino acids. A few of these amino acids make up an active site. This is a cavity in which other molecule(s) can bind; this molecule is known as the substrate. The substrate is held in the active site by hydrogen bonds that temporarily form between the hydrophilic R-groups of the active site and on the substrate molecule. The structure that results is the enzyme-substrate complex. The compounds on which the enzymes act upon are called substrates. The enzyme could...

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