Influence of pH on the activity of potato Catalase
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| Submitted: Sun Dec 15 2002
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Investigation 6.4 Influence of pH on the activity of potato Catalase Introduction Catalase occurs in many plant and animal tissue. It breaks down toxic Hydrogen peroxide, formed as a by-product of various biochemical reactions, into water and oxygen. The activity of most enzymes is influenced by changes in pH. In this experiment, potato discs in solutions of known pH act on hydrogen peroxide, and the rate at which oxygen is evolved is measured. This reflects the activity of the Catalase in the potato. Procedure Use a cork borer to cut cylinder shapes from a potato, use the cork borer to the diameter of 1cm. Place a ruler beside the potato cylinder and cut the potato into discs that are 1mm thick. Place the discs straight into a petri dish filled with water, you need to cut around 60 discs. To measure the rate of...


