Logged out
Access All our Essays in an Instant
Swap your work for FREE access, or pay £4.99 for instant access
Central Experiment: The Action Of An Enzyme
Member rating: No Rating | Words: | Submitted: Sat Mar 26 2005
Page Preview
On the left is an image preview of every page of this document, and below are the first 150 words with formatting removed:
Enzyme Experiments Central Experiment: The Action Of An Enzyme, e.g., amylase converting starch to reducing sugar. 1. Substrate: starch - use the same volume of the same starch solution each time 2. Enzyme: amylase - use the same volume of the same amylase solution each time 3. Only use starch and amylase solutions that test negative for reducing sugar with Benedict's Reagent 4. Four test tubes individually one third full of (a) starch solution, (b) amylase solution, (c) starch solution and (d) water 5. Warm all to 37°C in a heated water bath at 37°C 6. Mix (a) and (b) - the experiment. Mix (c) and (d) - the control 7. Use a buffer to maintain the pH at a constant suitable value: pH 8 8. Every minute test a small sample of each for starch using iodine 9. Control Results: always blue-black - starch always present, starch not broken down 10....
Get instant access
- Instant, unlimited access to our documents in full
- Swap your work for free access, or pay £4.99
- To see the full version of this document and 147,231 others
Receive email updates for this category
- Simply tell us your email address and receive a weekly Study Help Email for FREE
- Receive 3 FREE essay views with each email
- Get all the latest essays from Coursework.Info & discussion from TheStudentRoom.co.uk
