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The effect of caffeine concentration on the heart rate of Daphnia.  

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The effect of caffeine concentration on the heart rate of Daphnia Aim To conduct an experiment into the relationship between the heart rate of a Daphnia and its exposure to various concentrations of caffeine. Method For this experiment we had: Culture of Daphnia in various concentrations (%) of caffeine (0.0, 0.0025, 0.005, 0.025, 0.05, 0.25, 0.5) Two cavity slides A pipette Stop watch Microscope Ice water Firstly, a job was designated to each member of the group and each had a counter, a recorder and a controller. The controller always set up each slide to ensure that the other members of the group were unaware of the concentration of caffeine that the Daphnia were exposed to, thereby eradicating bias, and also controlled the time. The counter would count the number of heart beats in ten second intervals by stabbing at a piece of paper with a pen, in an 'S' shape. This allowed for easy counting and...

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