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The Effect of Substrate on Respiration Rate in Yeast Aim To determine the effect different substrates; glucose, sucrose and lactose, have on the respiration rate of yeast. Scientific Background Living organisms require energy from ATP to carry out many functions such as active transport. ATP is formed from joining ADP and a phosphate ion together and this process requires energy which it gets from respiration. Monosaccharides can enter at different stages of respiration but disaccharides cannot enter respiration without first being broken down into monosaccharides. Glucose is a monosaccharide and enters at the beginning of respiration but sucrose and lactose are both disaccharides and must be broken down. Sucrose is broken down into glucose and fructose and lactose is broken down into glucose and galactose. These all break down into a glucose molecule and another monosaccharide which enters lower down the process of respiration. Although sucrose can be broken down readily by yeast, lactose can not...

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