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Investigate the effect of changing temperature on the rate of anaerobic respiration.
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Josie Richards 13NB Investigating the effect of temperature on the rate of anaerobic respiration Aim: To investigate the effect of changing temperature on the rate of anaerobic respiration. To do this, I plan to use the simple procedure of counting the number of bubbles a boiling tube of respiring yeast produces over a period of time. Prediction: I predict that the number of bubbles given off will increase as the temperature is increased up to 40oC. After this point I would expect to very few or no bubbles produced at all. At the lower temperatures, I would also expect there to be few bubbles produced (i.e. between 0 and 10oC) Apparatus: * Yeast suspension in 10% glucose * Water bath (250ml beaker) * Thermometer * 10ml syringe * 2 x boiling tubes * Diazine green * Delivery tube and bung * Liquid paraffin * Stop clock * Test-tube rack * Ice * Hot water Method: 1. Place 20cm3 yeast/glucose mixture in boiling tube 2. Add a few...
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