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'Does Caffeine Affect Heart Rate In Daphnia?' Effect of Caffeine on Heart Rate in Daphnia TITLE 'Does caffeine affect heart rate?' HYPOTHESIS I predict that the water fleas heart rate will increase as caffeine is added. METHOD The Investigation was carried out in the following way: * A few strands of cotton wool were placed on a cavity slide (this helped restrict the movement of the water flea) * One large water flea was put on a cavity slide using a pipette * Water was removed from around the water flea using filter paper * One-Two drops of distilled water was added (This helped maintain sufficient oxygen supply to the flea) * To keep the temperature in control, a cavity slide was placed under the slide to minimise the amount of heat and light going through to the microscope * Water flea was viewed under low power * Focus was paid to its heart which could be seen through its translucent body * Stop watch...

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