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Practical Method Title "An experiment to investigate the rate of anaerobic respiration of yeast in various respiratory substrates." In this experiment, the independent variable is various different respiratory substrates being used (glucose, sucrose, maltose, lactose) and the dependent variable is the rate of respiration (measured by movement of manometer fluid which moves in relation to the amount of carbon dioxide released). Apparatus Yeast Glucose Sucrose Maltose Lactose pH7 buffer Top pan balance Stopwatch Thermometer Manometer fluid Capillary tube 20ml & 1ml syringe 100ml beakers Distilled water Water trough Kettle Stirring rod Stopwatch Spatula Background information My investigation will involve analysing how yeast respires in various different substrates: Glucose, Lactose, Maltose and Sucrose. All four of these respiratory substrates are carbohydrates. Glucose Glucose is a monosaccharide sugar, which is a 'simple sugar' that have between 3 and 10 carbon atoms per molecule. They are sweet and all soluble in H2O. It has the chemical composition C6H12O6. Glucose is a white crystalline solid but is less sweet then ordinary table sugar. Powdered dry glucose exists mainly in straight chain...

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