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To examine the effects of various physio-chemical treatments upon the permeability of beetroot cells.
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- Words:
- 586
- Submitted:
- Mon Nov 24 2003
... Toyin Dawudu Biology LBio1 October 03 Aim: To examine the effects of various physio-chemical treatments upon the permeability of beetroot cells. Hypothesis: The betacyanin pigment of beet roots is normally sequestered in the vacuole and, by means of the properties of the tonoplast and cell membrane, does not leak into the cytosol or the extra-cellular sap of the beet root. Of course if the beet root is cut cells are sliced open and the pigment spills out, but if the membrane is altered (phospholipid bilayer + proteins) more subtly leakage (diffusion) of betacyanin is induced. (Reference to www.mrothery.co.uk/cells/resources/betacyanin.doc) Apparatus: Take care using a cork borer. * Cork borer * White tile * Knife * Pens * Ruler * Water baths at 20, 40, 60, 80oC * Measuring Cylinder * 2 boiling tube racks * Crushed ice * 8 boiling tubes * Thermometer (one per water bath) * Colorimeter * Cuvettes * Stopclock * Distilled water * Pipette for measuring 2 cm3 Variables: There are different types of Variables, there are: 1. Controlled Variable 2.
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