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Study the effects of temperature on the movement of the red pigment Anthocyanin through the vacuole of a beetroot.  

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Hypothesis: In this experiment I aim to study the effects of temperature on the movement of the red pigment Anthocyanin through the vacuole of a beetroot. Under normal conditions, the movement of Anthocyanin across the plasma membrane is zero. As temperatures increase until around 40?C, there will be no change in the amount of Anthocyanin movement across the vacuole membrane. However, as temperature increases past around 45?C, Anthocyanin will start to 'leak' out of the vacuole. This is because the 4 bonds holding the all the membrane proteins in its tertiary structure will be broken. These bonds are the Hydrogen bonds, Peptide, disulphide bonds and hydrophobic interactions. They bonds break due to the greater kinetic energy (k.e) found in the membranes bi-lipid layer. This in turn leads to the fluid mosaic model becoming more animated and so the Hydrogen bonds break, this in turn breaks the other bonds holding the channel proteins...

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