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Stomata Investigation The following experiment included an investigation into how the location of a leaf from a specific plant may effect its density of stomata on the lower epidermis. Stomata are one of the internal factors, which affect the rate of transpiration. It was hypothesised that leaves on the outer edge of a plant or tree would have a higher density of stomata due to environmental factors such as availability of light and air movement. The leaves used for the experiment were taken from a Holly tree, biologically referred to as Ilex. Materials: * Ilex- taken for experimental purposes ( 5 exterior and 5 interior) * Forceps- to remove impression * 10 Microscope slides and cover slips- for viewing under microscope * Video Microscope monitor- to observe and count stomata * Labels- for identification * Graph paper- outline drawing of leaf samples * Stage micrometer- measure the field view through microscope eyepiece * Pencil, Calculator, Ruler Method: 1. Materials are laid out on...

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