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Pigment Chromatography Lab Sahar Rudgar Mr. Wright SBI4U- Per. 4 Tuesday Mar. 22nd, 2005 Purpose: Separation of plant cell pigments by paper chromatography. Materials: * Chromatography paper * Scissors * Test tube * Chromatography tube and solvent * Pencil * Fresh leaf Procedure: 1.) A test tube was obtained containing developing fluid (9 volumes of petroleum ether / 1 vol. acetone) and a stopper with a hook. 2.) A 15 centimeter strip of Whatman chromatography strip was cut. 3.) One end to a point was trimmed with scissors. A pencil line was drawn just above the point. 4.) The strip on a hard clean flat surface was laid. A fresh leaf was put over the paper and the tissue was crushed onto the pencil line by rolling the edge of a test tube along the leaf surface. The leaf was moved and this was repeated several times. The line of pigment which has been crushed onto the paper was kept as thin as possible. 5.) The paper...

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