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My aim is to extract the various photosynthetic pigments from a grass tissue and later to separate them and determine which one is which by using thin layer chromatography.
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Klaudia Stadnicka Due 28.June.2002 Biology; IB5 LAB REPORT #7 CHROMOTEMOGRAPHIC SEPERATION OF PLANT PIGMENTS AIM: My aim is to extract the various photosynthetic pigments from a grass tissue and later to separate them and determine which one is which by using thin layer chromatography. To calculate Rf values for all of the pigments. Hypothesis: I expect to observe various pigments extracted. There will be a distinction between them and separation will occur due to the pigments' different solubility and attraction to the static phase. Prediction: I predict that separation of each of the pigments will occur and will be able to be observed. I also predict that the pigment that will travel the closest is chlorophyll b, and then chlorophyll a, followed by xantophyll and at the end there will be cartenoid pigments. Variables: There are no variables in this specific lab, because it is an observation lab, where we are meant to observe separation of pigments. METHOD: Equipment: * A few grass...
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