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Enzyme Specificity Experiment. Aim To observe whether enzymes are specific and if so, which ones work best with yeast-therefore ferment. Hypothesis Sugar -> ethanol + carbon dioxide + energy C6H12O6 -> 2C2H5OH + 2CO + energy The sugar (substrate) used in this fermentation can vary. This experiment uses the amount of CO2 evolved to determine which sugars yeast enzymes can use. Materials * Distilled water * Glucose * Galactose * Fructose * Sorbose * Yeast Apparatus * Fermentation tubes x5 * Test Tubes x5 * Pipettes x5 * Temperature controlled water bath at 30°C * Ruler Diagram Method For the control, 10 drops of distilled water were placed in a fermentation tube along with 10 drops of yeast. This fermentation tube was then topped-up to the brim with more distilled water. The fermentation tube was then place upside down in water filled test tubes and the level of the fluid was recorded. After the level of the fluid was recorded and the other tubes completed...

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