Effect of temperature on catalase
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Aim and Hypothesis The investigation that we have chosen to do is how the effect of temperature affects the rate of reaction of hydrogen peroxide to water and oxygen using the enzyme catalase. I predict that the higher the temperature the faster the rate of reaction will be and the more oxygen there will be given off. I've based this prediction on kinetic theory (every 10 degree rise in temperature the rate of reaction doubles.) This is because the substrate will lock on twice as fast, as it is travelling twice as fast. To back up this prediction a pre experiment was carried out, one result was obtained, using this result here is a table of predicted values: Temp of 2H2O °C Amount of Oxygen produced CM3 7 3.5 17 7 27 14 37 28 47 56 57 112 67 0 - Denatured Red = initial experiment average. * Note: although most enzymes denature above the bodies natural temperature (35 - 37 degrees) catalase denatures at around 60 degrees. * When...


