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EFFECT OF TEMPERATURE ON THE MOVEMENT OF PIGMENT THROUGH CELL MEMBRANE INTRODUCTION: AIM: The aim of experiment is to prove that temperature effects on a proteins in cell membranes so that pigment can pass through them HYPOTHESIS: On higher temperatures proteins in membranes denature so there is no barrier to prevent the passage of large molecules THEORETICAL BACKGROUND: Certain chemicals and treatments, such as ethanol or high temperatures, can destroy the partial permeability of cell membrane. The membranes are still present but behave as if holes have been punched through them and they no longer provide barrier to the passage of large molecules such as sucrose. High temperatures and alcohols denature membrane proteins and increase fluidity of membrane lipids; alcohols at high concentrations can also dissolve lipids. In beetroot cells the red anthocyanin pigment occurs in the vacuoles. Each vacuole is surrounded by a tonoplast membrane and outside of it, the cytoplasm is surrounded...

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