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Anexperiment to investigate the effect of temperature on the leakage ofAnthocyanin from beetroot tissue
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An experiment to investigate the effect of temperature on the leakage of Anthocyanin from beetroot tissue My aim for this experiment to determine the effect of temperature on the leakage of Anthocyanin from the plasma membrane of a beetroot. I think that as the temperature increases the beetroot will start leaking out more Anthocyanin because the cell membrane which consists of proteins and lipids is affected by heat. So I predict that as the temperature increases so the amount of Anthocyanin leakage will also increase. To do my experiment I have chosen to use these temperatures: 30°, 40°, 50°, 60°, 70°C. I have chosen these temperatures because I estimate that at around 50°C the proteins tertiary structure will begin to denature and change, causing the Anthocyanin to leak out of the cells vacuole. So by using these temperatures I have a wide scope to see what happens around this...
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