Absorption Spectrum of Chlorophyll.
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Absorption Spectrum of Chlorophyll Group R1 Indradeo Hemraj Craig Rineer Sushmitha Kurapati Mariza Clement BE 210 Spring 1997 The absorption spectra of chlorophyll from spinach leaves and okra pods were determined along with the relative amounts of chlorophyll a and chlorophyll b in each of the two plants. The chlorophyll was extracted using 100% acetone and the absorption spectrum was determined over a range of 400-700 nm using a Spectronic 20D spectrophotometer. The relative amounts of chlorophyll a and chlorophyll b were determined using equations from the literature based on the absorbance data. Plots of absorbance versus wavelength were generated and the location of the peaks determined the wavelengths at which the chlorophyll extracts maximally absorbed. The chlorophyll extracts from both spinach and okra had two absorbance peaks. The mean absorbance maxima for spinach were (430 ? 3) nm and (662 ? 3) nm while those of okra were (430 ? 4) nm and (664 ? 4) nm....


