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The demonstration of the main stages of mitosis altered by the growth of broad bean seedlings in varied concentrations.  

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Hannah West 2313 Biology Practical Preparation Aim The demonstration of the main stages of mitosis altered by the growth of broad bean seedlings in varied concentrations. Method There are two stages to the preliminary experiment to this experiment. The first stage is the preparation of the roots used in the second part of the experiment. First stage: * 5 broad bean seedlings - roots intact (soaked and allowed to germinate for 7 days between damp cotton wool) * 5 test tubes * 5 varying concentrations of nutrients and phosphates * A test tube rack * Cotton wool Arrange like so: Arrange the 5 test tubes in a test tube rack and leave for 24 hours: Stage 2: * 5 prepared broad bean seedlings * 10cm3 of 50:50 concentrated hydrochloric acid and absolute alcohol * 10cm3 of 45% glacial acetic * 100cm3 of 70% alcohol * Acetic orcein in a dropping bottle * Microscope with a light source * Stop clock * Scissors * Watch glasses with glass covers * Forceps * Mounted...

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