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The advantages and limitations of electron microscopy There are two main branches of microscopy that are pertinent to cell biology. These branches arise from the two types of microscope; the light microscope and the electron microscope. The basic principles of light microscopy have been known since circa 17th century, however improvements in lens manufacture in circa 19th century allowed the use of microscopy to be much more practically available and useful. This is increased ability inspired rapid research into both the design of microscopes and the preparation of specimens. However, the light microscope can only magnify objects bigger than 0.2 micrometres; due to its limited resolving powers. This is because it utilises a beam of light. Relatively, light has a long wavelength, this means that when there are two small points close together there is too much refraction and wave front overlap, the eye then only sees one point. This can also...

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