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How the transmission and scanning electron microscopes work?
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How the transmission and scanning electron microscopes? Introduction Electron microscopy is an important tool in biology in general and cellular biology in particular. The two most important types of electron microscopes are the Transmission Electron Microscope (T.E.M.) and the Scanning Electron Microscope. (S.E.M.) these two types of microscopes are both used in medical, biological and material research, and both have advantages and disadvantages over each other, which I will be mentioning in this essay. The transmission electron microscopes (T.E.M.) The preparation for the T.E.M. Firstly the specimen is fixed, in other word it is preserved in as close to its living state as possible. The specimen then has to be dehydrated with an alcohol (e.g. ethanol). This is because the T.E.M. operates under a high vacuum and it is therefore no water should be to enter the microscope, to prevent this to happen the specimen is placed in liquid resin (similar to...
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