Electron Microscopy
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Electron Microscopy The electron microscope was first made when biologists found a problem with the resolution of a light microscope. Resolution means "the ability to distinguish between two points"; the problem is that the maximum resolution of a light microscope is 200nm. So if two points are closer together than 200nm they are seen as one. One example of this problem is that under a light microscope the centrioles in an animal cell appear as one, however when viewed under an electron microscope we can see that there are two centrioles at right angles to each other. Magnifying the image or using a higher magnification cannot overcome this problem, as we increase the magnification we do not get a better resolution just an enlarged image. In fact if we increase the magnification beyond a point it only decreases the resolution. The light a light microscope uses is between 400nm wavelength...

