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Standard addition was used to accurately quantify for quinine in an unknown urine sample containing approximately 100 ìg cm‑³ of quinine.
Member rating: No Rating | Words: | Submitted: Mon Dec 22 2003
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Instrumental Methods of Analysis Determination Of Quinine In Urine By Fluorescence Spectroscopy Name: Andrew Holmes, Tana Epsom and Rachel Nicholls Course: BSc Forensic Science Year: 2 Unit: Instrumental Methods of Analysis Date: 09.10.02 Summary Standard addition was used to accurately quantify for quinine in an unknown urine sample containing approximately 100 µg cm-³ of quinine. The fluorescence intensity of each standard addition solution was measured using a fluorescence spectrophotometer. The result was found to be that the unknown urine sample contained a concentration of 121 µg cm-³ of quinine. This was found by extrapolating the data on the standard addition graph of the variation in fluorescence with added quinine sulphate concentration. Aims: * To determine the variation in fluorescence with quinine sulphate concentration of standard addition solutions. * To determine the quinine sulphate concentration of an unknown urine sample. * To assess the data and judge whether other components of the urine interfere. * To adhere to all safety regulations required when...
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