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Spectrophotometer Lab Introduction: A spectrophotometer is an instrument that measures the quantitative amounts of light of different wavelengths absorbed and transmitted by a pigment solution (Mitchell, Reece). The spectrophotometer includes a light bulb, a reflector, and a detector. When a sample is in place and the chamber lid is closed, light from the light bulb passes through it. The detector measures the amount of light transmitted. The white light from the bulb is separated into different wavelengths by reflecting the beam off of a diffraction grating, forming a spectrum. A mirror is used to control the angle at which the light is reflected, so light of a single wavelength can be passed through the sample. A pigment solution (such as spinach and acetone) is exposed to different wavelengths of light to determine the points of the highest absorbance and transmittance percentages. (Hickey, Mary Kay). For this experiment, a Bausch and...

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