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To determine which solvent system gives the best resolution of separation of the individual amino acids.
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Ratio of volumes (Methanol: Chloroform: Water) Ds* /cm Alanine Aspartic Acid Leucine Glycine Alone In Mixture Alone In Mixture Alone In Mixture Alone In Mixture Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm 8:1:1 14.30 9.10 0.63 8.80 0.62 6.10 0.43 5.70 0.40 11.40 0.80 11.30 0.79 6.20 0.43 5.70 0.40 1:8:1 1:1:8 6:2:2 12.40 8.20 0.66 7.80 0.63 4.80 0.39 4.70 0.38 11.00 0.89 10.80 0.87 5.50 0.44 4.70 0.38 2:6:2 2:2:6 4:3:3 8.80 7.50 0.85 7.60 0.86 7.30 0.83 7.50 0.85 8.30 0.94 8.00 0.91 6.60 0.75 6.40 0.73 3:4:3 3:3:4 Key: Ds represents the distance moved by the solvent up the chromatography paper Da represents the distance moved by the individual amino acid up the chromatography paper Rf represents the Rf value of the individual amino acids, that is, (Rf = Da / Ds) The asterix, * indicates the uncertainty in the values calculated, which is, + 0.05cm. Ratio of volumes (Methanol: Ammonia: Water) Ds* /cm Alanine Aspartic Acid Leucine Glycine Alone In Mixture Alone In Mixture Alone In Mixture Alone In Mixture Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm Da* /cm Rf* /cm 8:1:1 8.80 6.40 0.73 - - 3.70 0.42 - - 7.50 0.85 - - 5.00 0.57 - - 1:8:1 1:1:8 6:2:2 13.40 10.50 0.78 - - 8.60 0.64 - - 11.70 0.87 - - 9.30 0.69 - - 2:6:2 2:2:6 4:3:3 9.60 8.80 0.92 8.50 0.89 8.60 0.90 8.50 0.89 0.89 0.93 - - 8.70 0.91 - - 3:4:3 3:3:4 Key: Ds represents the distance moved by the solvent up the chromatography paper Da represents the distance moved by the individual amino acid up the chromatography paper Rf represents the Rf value of the individual amino acids, that is, (Rf = Da / Ds) The asterix, * indicates the uncertainty in the values calculated, which is, + 0.05cm. Bases of the evaluation: To determine which solvent system gives the best resolution of separation of the individual amino acids, the following points...
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