To Identify Bio chemicals in an unknown substance
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| Submitted: Fri Jun 03 2005
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To Identify Bio chemicals in an unknown substance Introduction The object of the experiment is to test a substance and identify the bio chemicals, which exist in that substance. Food tests are used to identify which bio chemicals exist in a substance. The substances characterised are in the tests are: carbohydrates, starch, lipids and peptide bonds. Method: The following tests were used to identify the relevant substances: Benedicts Test Carbohydrates - reducing sugars such as glucose fructose and maltose * Add 2cm3 of a solution to a test tube. * Add an equal volume of Benedicts solution. * Shake and bring gently to the boil, shaking continuously to minimise spitting. Red brown precipitate result indicates reducing sugars present Disaccharide hydrolysis test and Benedicts test Carbohydrates - non-reducing sugars such as sucrose * Add 2cm3 of solution to a test tube . * Add 1cm3 dilute hydrochloric acid. * Boil for one minute. * Carefully neutralise with sodium hydrogen carbonate *...

