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The objective of this experiment is to determine the vitamin C content of a lemon.
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| Submitted: Wed Aug 27 2003
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Aim: The objective of this experiment is to determine the vitamin C content of a lemon. Apparatus: Lemon juice, vitamin C solution (fresh, 0.1%), 2 test tubes, test tube rack, pipette to measure 2.00 cm3 volume, DCPIP (dichlorophenolindophenol, 0.1% aqueous solution). Procedure: The lemon juice and vitamin C solution were provided by the teacher. The vitamin C solution was made up to contain 0.001 g vitamin C in 1.0 cm3 water. (i) Using a pipette, place exactly 2.00cm3 of DCPIP solution into a test tube. (ii) Using a different graduated pipette, add the vitamin C solution, drop by drop, to the DCPIP solution in the test tube. Shake the tube very gently after the addition of each drop of the vitamin C solution and continue adding drops until the DCPIP solution is decolorized. (iii) Record the exact volume of the vitamin C solution which has been added. (iv) Repeat this procedure. Average the results to...
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