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Paper Chromatography Objective To illustrate the use of paper chromatography for the separation and identification of amino acids. Results Amino Acid Distance travelled /cm Rf value by solvent by amino acid Aspartic acid - alone - in mixture 5.3 5.3 1.2 1.2 0.2264 0.2264 Leucine - alone - in mixture 5.3 5.3 3.95 4.3 0.7453 0.8113 Lysine - alone - in mixture 5.3 5.3 3.1 3.3 0.5849 0.6226 Q & A 1.> For each amino acid, compare your two Rf values with each other and with the specimen results (you can obtain from your teacher) Why do you think there is some variation? [ANS] Both Rf values are the same for aspartic acid but there is some variation for leusine & lysine. This may due to: 1.> The grease on our fingers contaminated on chromatographic paper will affect greatly on the amino acids that are less polar. 2.> The measurement error of the distance travelled by solvent and/or amino acid. 3.> The shape of chromatographic paper is not exactly square-shaped, so that one side of the paper may...

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