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Identifying amino acids by chromatography.
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| Submitted: Tue Nov 04 2003
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Identifying amino acids by chromatography Apparatus * Empty glass coffee jar * Lid for glass jar * Strip of chromatography paper * Capillary tubes * Staples * Oven * Fume cupboard * Ninhydrin solution * Lysine * Leucine * Valine * Glycine * Threomine * X acid * Y acid * Solvent- 4 parts butan-1-01, 1 part ethanoic acid and 1 part distilled water. Method * Fetch and set up equipment. * Pour a small quantity of the solvent into the glass jar. * Cover tank with lid. * On the chromatography paper, make a light pencil mark across the paper 2 cm's from one end. * Using pencil, place the names of the acids on the paper so you can identify them afterwards. * Practice using the capillary tubes on normal filter paper * Using the capillary tubes, place a small amount of each acid on its position along the line on the filter paper. * Make the spots no bigger than 2 mm's if possible. * Let the paper dry for a few minutes...
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