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Chromatography experiment.
Member rating: No Rating | Words: | Submitted: Wed Oct 08 2003
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20/10/01 Chromatography Experiment by Emma Walters Aim: To identify different unknown amino acids, within given substances. Apparatus: List 1 List 2 * 2 test tubes * Test tube rack * 2 bungs * 2 pins * 5 known amino acids: * Proline * Leucine * Lycine * Asparagine * Arginine * 1 unknown amino acid * Paper Chromatography * Paper Solvent * Pencil * Ruler * Tweezers * Paper Towel * Pipette * Ninhydrin * Incubator * Scissors * 1 Jam Jar * Treated Orange Juice * Untreated Orange Juice * TLC * TLC Solvent * Pencil * Ruler * Tweezers * Pipette * Paper Towel * Ninhydrin * Incubator Diagram 1: Diagram 2: Method (Part 1): * Cut 2 strips of paper chromatography using tweezers and scissors while resting on paper towel * Measure 1cm from bottom of strip and draw a line across * Draw a cross in the centre of this line * Attach a pin to the top of the chromatography paper, and put pin in bung * Place inside test tube, and measure 5mm from where the bottom of the paper lies, draw a line across the test tube *...
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