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Biology Chromatography Investigation Results Table of results to show RF values of amino acids found in egg white following dissection by trypsin. RF value = Distance moved by spot_____ Distance moved by the solvent Sample Cm Distance moved Cm moved by solvent RF Value Identity of amino Alanine 4.9 16.3 0.30 Leucine 11 16.3 0.67 Egg white A) 5.2 16.3 0.31 B) 11.2 16.3 0.68 Amino acid RF Value Lysine 0.14 Arginine 0.20 Aspartic Acid 0.24 Glycine 0.26 Serine 0.27 Glutamic Acid 0.30 Threonine 0.35 Alanine 0.38 Proline 0.43 Tyrosine 0.45 Methionine 0.55 Valine 0.60 Phenylalanine 0.68 Isoleucine 0.72 Leucine 0.73 RF table Class Data Sample 1 2 3 4 5 6 Alanine 0.3 0.3 0.3 0.3 0.3 0.3 Leucine 0.7 0.7 0.7 0.7 0.7 0.7 Egg white A 0.3 0.3 0.3 0.3 0.3 0.3 B 0.7 0.7 0.7 0.6 0.6 0.7 Get a piece of chromatography paper and draw a pencil line 4cm from the bottom. Dispense the egg white, alanine and leucine equally spaced along the line with a micropipette. Pour solvent in bottom of jar up to 1cm place paper in jar ensuring the solvent doesn't cover the pencil line (line of origin). Place lid on and leave in fume cupboard for 8-16 hours. Draw a line were solvent reached. The amino acids should show as purple spots. To calculate the RF values divide the distance traveled by...

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