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Analysis of sulphur dioxide content in wine
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- Words:
- 1042
- Submitted:
- Thu Nov 05 2009
- Mark submitted by Author:
... Analysis of sulphur dioxide content in wine Objective To determine the amount of sulphur dioxide, an antioxidant, present in wine by using volumetric analysis. Principle All free SO2 molecules is first convert into SO32- by NaOH solution: SO2 + 2OH- SO32- + H2O Acidification of the solution liberates all SO2: 2SO32- + 2H+ 2SO2 + 4H2O which is then titrated with 0.0057M iodine solution in which starch is used as end-point indicator: SO2 + I2 + 2H2O 2HI + H2SO4 Chemicals white wine (carbonated), 1M NaOH, 2M H2SO4, 0.0057M I2, starch solution Apparatus volumetric apparatus, pipette, measuring cylinder, dropper, white tile Procedure 1.> Find out the volume of wine from the label on the bottle. 2.> Pipette 25cm3 of white wine into a conical flask. 3.> Add about 12cm3 of 1M NaOH and stand for about 15 minutes. 4.> Add about 10cm3 of 2M H2SO4 to the mixture and then few drops
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