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An Investigation using Chromatography to determine the different Amino Acids

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An Investigation using Chromatography to determine the different Amino Acids, which are contained in both a Chicken and Duck egg and to establish the difference between them. I am going to do an experiment to find out which amino acids are contained in both a chicken egg and a duck egg. I will do this using chromatography, I will then use a equation which is relevant to my experiment, to calculate how far the amino acids have moved towards the solvent front, thus letting me compare with a table to see which amino acids are present. Equation Rf = x y Equipment Chicken egg Gas Jar Ninhydrin Chromatography Paper Gas Jar Lid Fume cupboard Pipette Double Sided Sellotape Hair Dryer A Solvent Butaniol - Glacial Ethanoic Acid - Distilled H2O With a ratio of 4:1:1 Method I will first need to prepare the egg white by separating it from the egg yolk and then adding the protease mixture to the...

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